Journal: International Journal of Nanomedicine

Article Title: Synergistic Neuroprotection of Fe 3 O 4 Nanoparticles Combined with Intermittent Theta Burst Stimulation in Ischemic Stroke via Nrf2-Mediated Ferroptosis Regulation

doi: 10.2147/IJN.S574711

Figure Lengend Snippet: Therapeutic mechanism of Fe 3 O 4 for precise ischemic stroke management.

Article Snippet: In vivo,The 4-8-week-old healthy male Sprague-Dawley (SD) rats were randomly divided into eight groups: 1) Sham group, 2) Saline group, 3) MCAO/R group, 4) iTBS group, 5) rTMS group, 6) Fe 3 O 4 +iTBS group, 7) Fe 3 O 4 +rTMS group, 8) Fe 3 O 4 +magnet group.

Techniques:

Journal: International Journal of Nanomedicine

Article Title: Synergistic Neuroprotection of Fe 3 O 4 Nanoparticles Combined with Intermittent Theta Burst Stimulation in Ischemic Stroke via Nrf2-Mediated Ferroptosis Regulation

doi: 10.2147/IJN.S574711

Figure Lengend Snippet: Fabrication and characterization of Fe 3 O 4 . ( A ) Representative TEM images of prepared NPs. ( B ) zeta potential measurements (n = 3). ( C ) Size distributions of Fe 3 O 4 by dynamic light scattering (n = 3). ( D ) Confirmation of magnetic targeting of Fe 3 O 4 by static magnetic field. Red circles indicate nanoparticle aggregation toward the magnetic field. ( E ) Fe 3 O 4 labeled with iR780 by fluorescence images. ( F ) Characterization of Fe 3 O 4 NPs using EDS.

Article Snippet: In vivo,The 4-8-week-old healthy male Sprague-Dawley (SD) rats were randomly divided into eight groups: 1) Sham group, 2) Saline group, 3) MCAO/R group, 4) iTBS group, 5) rTMS group, 6) Fe 3 O 4 +iTBS group, 7) Fe 3 O 4 +rTMS group, 8) Fe 3 O 4 +magnet group.

Techniques: Zeta Potential Analyzer, Labeling, Fluorescence

Journal: International Journal of Nanomedicine

Article Title: Synergistic Neuroprotection of Fe 3 O 4 Nanoparticles Combined with Intermittent Theta Burst Stimulation in Ischemic Stroke via Nrf2-Mediated Ferroptosis Regulation

doi: 10.2147/IJN.S574711

Figure Lengend Snippet: Study on BBB penetrability, biodistribution and biosafety of Fe 3 O 4 NPs. ( A ) Hemolysis test of Fe 3 O 4 NPs in different concentrations. ( B ) Fluorescence images of the heads of MCAO/R rats after intravenous (iv.) injection with iR780-labeled NPs at 6 h. ( C ) Fluorescence images of brain sections at 0, 24, 48, 72, 96 and 120 h post-injection. Fluorescence signals were quantified using Living Image software with background subtraction. Data represent transient tight junction remodeling and enhanced permeability, and no statistical analysis was performed. ( D ) Radiant efficiency was quantified at 0, 24, 48, 72, 96, and 120 h post-injection. Data represent a single subject. ( E ) Fluorescence images of the dissected main organs and brains at 48 h post-injection. ( F ) Representative H&E staining of ischemic brain sections after different treatments. Scale bar = 20 μM. ( G ) Prussian blue staining of tumors after iv. injection 3 days of Fe 3 O 4 NPs. Scale bar: 20 μM and ( H ) their quantitative analyses (n = 5). Data are presented as means ± SD (n = 5). Statistical significance was determined by one-way ANOVA with a Tukey post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: In vivo,The 4-8-week-old healthy male Sprague-Dawley (SD) rats were randomly divided into eight groups: 1) Sham group, 2) Saline group, 3) MCAO/R group, 4) iTBS group, 5) rTMS group, 6) Fe 3 O 4 +iTBS group, 7) Fe 3 O 4 +rTMS group, 8) Fe 3 O 4 +magnet group.

Techniques: Fluorescence, IV Injection, Labeling, Injection, Software, Permeability, Staining

Journal: International Journal of Nanomedicine

Article Title: Synergistic Neuroprotection of Fe 3 O 4 Nanoparticles Combined with Intermittent Theta Burst Stimulation in Ischemic Stroke via Nrf2-Mediated Ferroptosis Regulation

doi: 10.2147/IJN.S574711

Figure Lengend Snippet: Neuroprotective effects and modulation of microglia phenotypes in vitro. ( A ) and ( B ) The cell viability of SH-SY5Y cells and BV2 cells after OGD/R or incubation with Fe 3 O 4 of different concentration for 12 h (n=2). ( C ) Fluorescence images of ROS in different groups after OGD/R (n = 3). Scale bar: 40 μM. ( D ) Immunofluorescence co-staining images of BV2 cells showing M1 phenotype marker CD86 (green) (n = 3). Scale bar: 20 μM, corresponding quantitative fluorescence of ROS ( E ) and CD86 (F). ( G ) The changes of Δ ψ m in SH-SY5Y cells undergoing OGD/R after different treatments for 48 h and ( H ) their quantitative analyses (n = 3). Relative expression of pro-inflammatory IL-6 ( I ) and TNF-α (J), and MDA ( K ) in DMEN from BV2 cell culture. Data are presented as means ± SD (n = 3). ***P < 0.001, **P < 0.01, *P < 0.05.

Article Snippet: In vivo,The 4-8-week-old healthy male Sprague-Dawley (SD) rats were randomly divided into eight groups: 1) Sham group, 2) Saline group, 3) MCAO/R group, 4) iTBS group, 5) rTMS group, 6) Fe 3 O 4 +iTBS group, 7) Fe 3 O 4 +rTMS group, 8) Fe 3 O 4 +magnet group.

Techniques: In Vitro, Incubation, Concentration Assay, Fluorescence, Immunofluorescence, Staining, Marker, Expressing, Cell Culture